اثر پیش تیمار اسپرمیدین و متیلن بلو بر برخی پاسخ‌های فیزیولوژیکی گیاه بابونه (Matricaria recutita L.) به تنش شوری

Abstract: Salinity is one of the most important factors that limit plant growth and production in the whole word. Compounds, which are able to reduce the damaging effects of various stresses such as salinity, should be of great important. In this investigation, the effects of spermidine on salt tolerance of Matricaria recutitaa were investigated. The results showed that, salt stress in chamomile plants caused the reduction of growth and increased the amounts of hydrogen peroxide, malondialdehyde and the activity of ascorbate peroxidase and guaiacol peroxidase. Pre-treatment of chamomile plants with spermimdine had no significant effect on shoot growth and the activity of ascorbate peroxidase enzyme, while reduced the content of hydrogen peroxide and malondialdehyde obviously, and increased the root growth and activity of guaiacol peroxidase significantly. Application of spermidine with methylene blue reduced the effect of spermidine on the increment of root growth, decrease of hydrogen peroxide, malondialdehyd and ascorbate peroxidase activity while had no significant effect on shoot growth and guaiacol peroxidase activity. Therefore, it seems that Spd effects on these physiological parameters are through nitric oxide and guanylate cyclase pathway however, more studies required for detail investigations.. .